Yes, Rublon MFA for Windows Logon and RDP supports Azure Virtual Desktop (AVD).
You can protect Windows sign-in on Azure Virtual Desktop session hosts by installing the Rublon MFA for Windows connector on the Windows virtual machines used in your AVD environment. Rublon MFA for Windows supports both Windows logons and RDP connections, works with physical and virtual machines, and supports Microsoft Entra ID (Azure AD) as an authentication source.
Helpful Links
Rublon MFA for Windows Logon and RDP - Documentation
Can Rublon MFA be used with Virtual Desktop Infrastructure (VDI)?
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