Yes, Rublon MFA can be used with Virtual Desktop Infrastructure (VDI), including but not limited to VMware Horizon and Citrix Virtual Desktops.
For example, the Rublon for Windows Logon and RDP connector supports Virtualization Software, enabling MFA for logins to Windows machines using such software.
Helpful Links
Does the Rublon for Windows Logon and RDP connector work with Virtualization Software, e.g., Hyper-V Server 2019?
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